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二氢R123，Dihydrorhodamine 123
Dihydrorhodamine 123
CAS#：109244-58-8
Chemical Name：Dihydrorhodamine 123
Dihydrorhodamine 123
|
Cat# |
Size |
Price |
MW |
Abs |
Em |
Soluble in |
Storage |
|
15206 |
10 mg |
$75 |
346.38 |
507 nm |
529 nm |
DMSO |
F/D/L |
|
|
Features and Biological ApplicationsDihydrorhodamine 123 is by far the most-used probe for measurement of intracellular H2O2. DHR 123 is oxidized directly to rhodamine 123, which is excitable at 488 and emits at 515 nm in the same emission range as FITC. It is widely used in human neutrophils, human eosinophils, HL60 cells, rat mast cells, guinea pig neutrophils, cultured chondrocytes, rat brain, rat renal proximal tubular cells, mesangial cells and L929 cells. In combination with other fluorescent reagents (such as surface receptor analysis by fluorescent antibodies, cell viability using propidium iodide, and calcium indicators) this probe can be used for multiplex measurements. |
References
1. Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J. (2007) Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD. Clin Chem, 53, 890.
2. Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C. (2004) Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123. J Fluoresc, 14, 17.
3. Wrona M, Patel K, Wardman P. (2005) Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals. Free Radic Biol Med, 38, 262.
4. Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA. (2003) Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol, 111, 374.
5. Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM. (1999) Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med, 27, 146.
6. Ischiropoulos H, Gow A, Thom SR, Kooy NW, Royall JA, Crow JP. (1999) Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123. Methods Enzymol, 301, 367.
7. Crow JP. (1997) Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide, 1, 145.
8. Sobreira C, Davidson M, King MP, Miranda AF. (1996) Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations. J Histochem Cytochem, 44, 571.
9. van Pelt LJ, van Zwieten R, Weening RS, Roos D, Verhoeven AJ, Bolscher BG. (1996) Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst. J Immunol Methods, 191, 187.
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Description
| Name | 二氢R123，Dihydrorhodamine 123 | ||
|---|---|---|---|
| CAT# | 15206-10 mg | CAS# | 109244-58-8 |
| Storage# | -20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 507 | Em(nm)# | 529 |
| MW# | 346.38 | Solvent# | DMSO |
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