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BCECF, AM [2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester]

BCECF AM is cell membrane permeable and is easily hydrolyzed by esterases to BCECF. BCECF is not cell membrane permeable, and it accumulates inside viable cells (Fig. 1). BCECF emits a strong green fluorescence; therefore, it is easy to visualize viable cells. BCECF-AM is also utilized as an intracellular pH indicator. The excitation and emission wavelengths of BCECF are 500 nm and 530 nm, respectively. Usually, over 80% of loaded BCECF will remain inside the cell for at least 2 hours.

零售价

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市场价

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浏览量:

1000

产品编号

21202-1mg/1250元

所属分类

Fluorescence reagents and more

数量

库存:



产品描述

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BCECF, AM

CAS#：117464-70-7

中文名：2',7'-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯

英文名：2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester

结构式：

Description:

BCECF, AM， 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester

Appearance: orange or orange-brown crystals
Purity: >90.0% (HPLC)
MW: 688.59, C₃₅H₂₈O₁₅

CAS#：117464-70-7
Storage Condition : -20^oC, protect from light
Shipping Condition : ambient temperature

Product Description

Application:

General Protocol (for Human Neutrophil)*
Reagents:
1 mM BCECF-AM/DMSO solution (1 mg BCECF in 1.45 ml DMSO)
HEPES buffer saline (20 mM HEPES, 153 mM NaCl, 5 mM KCl, 5 mM glucose, pH 7.4)
Protocol:
1. Suspend cells in HEPES buffer solution to prepare 4x107 cells per ml.
2. Add 1 mM BCECF-AM/DMSO solution to the cell suspension to prepare 3 μM BCECF-AM (1/300 vol of cell suspension) as the final concentration.
3. Incubate the cell suspension at 37 oC for 30 min.
4. Wash the cells 3 times with HEPES buffer saline and then prepare 3x106 cells per ml of the cell suspension.
5. Determine the fluorescence intensity using a fluorescence microscope or a confocal laser microscope coupled with an image analyzer.

* Cell staining conditions differ by cell type, so it is necessary to optimize the conditions for each experiment.

Reference
S. A. Weston, et al., J. Immunol. Methods, 133, 87 (1990);
S. A. Weston, et al., Cytometry, 13, 739 (1992);
L. S. De Clerck, et al., J. Immunol. Methods, 172, 115 (1994);
V. Radosevic, et al., Cytometry, 20, 281 (1995);
P. Franck, et al., J. Biotechnol., 46, 187 (1996).
M. Ikuma, et al., J. Gerontol. A Biol. Sci. Med. Sci., 51, B346 (1996);
A. Sachinidis, et al., Br. J. Pharmacol., 119, 787 (1996);
T. Hirohashi, et al., J. Pharmacol. Exp. Ther., 280, 813 (1997);
D. W. Johnson, et al., Am. J. Physiol., 272, F484 (1997);
B. M. Wolska, et al., J. Mol. Cell. Cardiol., 29, 2653 (1997);

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	BCECF, AM [2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester]
CAT#	21202-1 mg	CAS#	117464-70-7
Storage#	-20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	505	Em(nm)#	520
MW#	808.69	Solvent#	DMSO

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BCECF, AM [2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester]

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