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Calcein, AM

Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to Calcein. After Calcein-AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell (Fig. 1). Among other reagents, including BCECF-AM and Carboxy-fluorescein diacetate, Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any cellular functions such as proliferation or chemotaxis of lymophocyte. In addition, viability assays using Calcein are reliable and correlate well with the standard 51Cr-release assay. The excitation and emission wavelengths of calcein are 490 nm and 515 nm, respectively.

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1000

产品编号

22002-1mg/1800元

所属分类

Fluorescence reagents and more

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

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Calcein, AM

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Description:

Chemical Name:3',6'-Di(O-acetyl)-4',5'-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein tetraacetoxymethyl ester
CAS: 148504-34-1
Storage Condition : -20^oC
Shipping Condition : blue ice

Appearance: white or slightly yellow crystals
Purity: >90.0% (HPLC)
MW: 994.86, C₄₈H₄₆N₂O₂₃

Product Description

Staining Procedure

1. Prepare 1 mM Calcein-AM solution with DMSO and dilute to prepare 1-50 μM Calcein-AM solution with PBS.a)

2. Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture.b)

3. Incubate the cell at 37 oC for 15-30 min.

4. Wash cells twice with PBS or an appropriate buffer.

5. Observe the cells under a fluorescence microscope with 490 nm excitation and 515 nm emission filters.

a) If the Calcein-AM has difficulty loading into cells, use a detergent such as Pluronic F127.
b) Or you may replace the culture medium with 1/10 concentration of Calcein-AM buffer solution.

Reference

S. A. Weston, et al., J. Immunol. Methods, 133, 87 (1990);
X. M. Wang, et al., Hum. Immunol., 37, 264 (1993);
R. Lichtenfels, et al., J. Immunol. Methods, 172, 227 (1994);
P. Rat, et al., Cell. Biol. Toxicol., 10, 329 (1994);
F. Braut-Boucher, et al., J. Immunol. Methods, 178, 41 (1995);

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	Calcein, AM
CAT#	22002-1 mg	CAS#	148504-34-1
Storage#	-20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	495	Em(nm)#	515
MW#	994.86	Solvent#	DMSO

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