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DAPI，UltraPure 超纯级

DAPI is an AT-sequence specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. Though DAPI is not permeable through viable cell membranes, it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photo-bleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

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1000

产品编号

17510-10mg/515元；100mg/3250元

所属分类

Fluorescence reagents and more

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产品描述

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DAPI and DAPI solution

Chemical Name: 4',6-Diamidino-2-phenylindole, dihydrochloride CAS: 28718-90-3
Appearance: yellow powder MW: 350.25, C₁₆H₁₇Cl₂N₅
Storage Condition : -20^oC, protect from light； Shipping Condition : ambient temperature

Product Description

Staining Procedure

1. The 1mM DAPI stock solution was prepared by adding 35mg DAPI powder into 100mL ddH2O.

2. Prepare 10-50 μM DAPI solution with PBS or an appropriate buffer.

3. Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture. Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.

4. Incubate the cell at 37^oC for 10-20 min.

5. Wash cells twice with PBS or an appropriate buffer.

6. Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.

Note: Since DAPI may be carcinogenic, extreme care is necessary during handling.

Reference

M. A. Hotz, et al., Cytometry, 15, 234 (1994); . Vollenweider, et al., J. Immunol. Methods, 149, 133 (1992);

N. Poulin, et al., Cytometry, 16, 227 (1994); F. Otto, et al., Stains Technol., 60, 7 (1985);

C. Souchier, et al., Cytometry, 20, 203 (1995); I. W. Taylor, et al., J. Histochem. Cytochem., 28, 1224 (1980);

J. Kapuscinski, et al., Biotech. Histochem., 70, 220 (1995); S. M. Bilinski, et al., Histochem. J., 28, 651 (1996);

S. Burde, et al., Cytometry, 25, 295 (1996); E. A. Moscone, et al., Chromosoma, 105, 231 (1996);

S. Saby, et al., Appl. Environ. Microbiol., 63, 1564 (1997); K. Kameyama, et al., Oncol. Rep., 6, 1345 (1999).

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *UltraPure grade*

Cat#	Size	Price	MW	Abs	Em	Soluble in	Storage
17510	10 mg	$49	350.25	358 nm	461 nm	DMSO	F/D/L

Features and Biological Applications

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 400 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.

References

1. Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM. (2006) Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads. Chromosome Res, 14, 673.

2. Gichner T, Mukherjee A, Veleminsky J. (2006) DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I. Mutat Res, 605, 17.

3. Suda J, Travnicek P. (2006) Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research. Cytometry A, 69, 273.

4. Chen JM, Hong YH, Wang YP, Bowley S, Wan JM. (2006) [Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L]. Yi Chuan, 28, 184.

5. Samatadze TE, Muravenko OM, Bol'sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV. (2005) [Investigation of chromosomes in varieties and translocation lines of pea Pisum sativum L. by FISH, Ag-NOR, and differential DAPI staining]. Genetika, 41, 1665.

6. Krishan A, Dandekar PD. (2005) DAPI fluorescence in nuclei isolated from tumors. J Histochem Cytochem, 53, 1033.

7. Barcellona ML, Gammon S, Hazlett T, Digman MA, Gratton E. (2004) Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes. Microsc Res Tech, 65, 205.

8. Pancheva EV, Volkova VN, Kamzolkina OV. (2004) [DNA quantification in nuclei of cultivated mushroom with DAPI staining]. Tsitologiia, 46, 381.

9. Daniel B, DeCoster MA. (2004) Quantification of sPLA2-induced early and late apoptosis changes in neuronal cell cultures using combined TUNEL and DAPI staining. Brain Res Brain Res Protoc, 13, 144.

10. Li M, Wu RS, Tsai JS. (2003) DAPI derivative: a fluorescent DNA dye that can be covalently attached to biomolecules. Bioorg Med Chem Lett, 13, 4351.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	DAPI，UltraPure 超纯级
CAT#	17510-10mg；17510-100mg	CAS#	28718-90-3
Storage#	−20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	358	Em(nm)#	461
MW#	350.25	Solvent#	Water

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