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碘化丙啶PI，Propidium iodide，UltraPure 超纯级

Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. Though PI does not permeate viable cell membranes, it passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.

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1000

产品编号

17515-10mg/180元；25mg/365元

所属分类

Fluorescence reagents and more

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产品描述

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PI，Propidium iodide，UltraPure

Name：3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridini- um diiodide；Propidium iodide；Propidium diiodide；PI

分子式：C27H34I2N4分子量：668.39；CAS#：25535-16-4

Description:

Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, aqueous solution
Product: 1.5 mM PI aqueous solution (1 mg PI per 1 ml water)
Appearance: Dark red powder； Orange or red solution in water
MW: 668.39, C₂₇H₃₄I₂N₄
Storage Condition : 0～5^oC, protect from light
Shipping Condition : ambient temperature

Product Description

Application:

Staining Procedure

1. Prepare 10-50 μM PI solution with PBS or an appropriate buffer.a)

2. Add PI solution with 1/10 of the volume of cell culture medium to the cell culture.b)

3. Incubate the cell at 37^oC for 10-20 min.

4. Wash cells twice with PBS or an appropriate buffer.

5. Observe the cells under a fluorescence microscope with 535 nm excitation and 615 nm emission filters.

a) Since PI may be carcinogenic, extreme care is necessary during handling.

b) Or you may replace the culture medium with 1/10 concentration of PI buffer solution.

Reference：

1. W. Taylor, et al., J. Histochem. Cytochem., 28, 1224 (1980);

2. W. M. J. Vuist, et al., Cancer Res., 49, 3783 (1989);

3. A. Pollack, et al., Methods Cell. Biol., 33, 315 (1990);

4. A. Bertuzzi, et al., Cell. Biophys., 17, 257 (1990);

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

注意事项：

Sigma的MSDS显示碘化丙啶PI与EB一样对生殖细胞致突变性，是致癌物质，请必须严格注意防护！

碘化丙啶PI是Germ cell mutagenicity生殖细胞突变物质；In vitro tests showed mutagenic effects体外实验显示有致突变性！

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	PI，Propidium iodide，UltraPure 超纯级
CAT#	17515-10mg；17515-25mg	CAS#	25535-16-4
Storage#	−20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	535	Em(nm)#	617
MW#	668.39	Solvent#	Water

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