中文 | EN
Hoechst 33258 UltraPure，超纯级
Hoechst®为北京富百科生物技术有限公司注册商标，未经授权严禁使用！
中文名：二苯甲亚胺, 三氯化氢2'-(4-羟基苯基)-5-(4-甲基-1-哌嗪基)-2,5'-二-1H-苯并咪唑
英文名：2’-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloride
别名：Hoechst 33258，bisBenzimide H 33258，HOE 33258bisBenzimide H 33258或HOE 33258
Description:
Chemical Name: 2’-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloride
Appearance: Yellow green powder
MW: C25H24N6O · 3HCl =533.88
Storage Condition : 0-5oC, protect from light
Shipping Condition : Ambient temperature
Product Description
Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.
Staining Procedure
1. First prepare the stock HOECHST Dye solution: 1.9 mM Hoechst 33258 aqueous solution (1mg HOECHST 33258 Dye Powder in 1 ml dH2O)
2. Prepare 10-50 μM Hoechst dye solution with PBS or an appropriate buffer to dilute the stock solution.
3. Add Hoechst dye solution with 1/10 of the volume of cell culture medium to the cell culture.
4. Incubate the cell at 37 °C for 10-20 min.
5. Wash cells twice with PBS or an appropriate buffer.
6. Observe the cells under a fluorescence microscope with 350 nm excitation and 460 nm emission filters.
a) Since Hoechst dyes may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of Hoechst dye buffer solution.
Safety warnings and precautions
NOTE: Qualified persons should always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！
产品参数
| Name | Hoechst 33258 UltraPure，超纯级 | ||
|---|---|---|---|
| CAT# | 17520-25mg；17520-100mg | CAS# | 23491-45-4 |
| Storage# | −20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 352 | Em(nm)# | 461 |
| MW# | 533.88 | Solvent# | Water |
The ultimate scientific research that strives for excellence is manifested in the details, forging the beauty of product quality.
Day after day, we persist in the pursuit of innovation. Through dedication, we experience and achieve a leading position in technology.
Room 208, 2nd Floor, Building 2, No. 4 Courtyard, Jinhang West Road, Shunyi District, Beijing
Customer Service hotline：400-686-3443
Email：info@fluorescence.cn