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SafeGreen®Nucleic Acid Gel Stain
2) Easy disposal: Safe to dispose in the drain
3) Good Compatibility: Spectrally compatible with all the existing instruments
4) High Sensitivity: Higher signal but lower background
5) High Stability: can be stored at RT and microwavable
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产品编号
011-500uL/支/518元
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0
关键词:
safegreen
凝胶
染色
电泳
琼脂糖
染料
核酸
1
产品描述
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SafeGreen®Nucleic Acid Gel Stain （10,000× in water）
SafeRed®, SafeView®, SafeStain®，DuGreen®和GelStain®are registered trademark and strictly forbidden to be used without authorization
Description
SafeGreen®(the ideal substitute for Sybr Green and 10000x GelGreen) is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). Our Ames test confirms that SafeGreen® is nonmutagenic at concentrations well above their working concentrations for gel staining. SafeGreen® are highly sensitive than EtBr either as precast gel stains or post gel stains.
SafeGreen® has UV absorption between 250nm and 300nm and a strong absorption peak centered around 500nm and is compatible with either a 254nmUV transilluminator or a gel reader equipped with visible light excitation(such as a 488nm laser-based gel scanner or a Dark Reader).
SafeGreen®Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated SafeGreen® solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.
Gel staining with SafeGreen® is compatible with downstream applications such as gel extraction and cloning. SafeGreen® is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Features
1) Relative Safety: Nonmutagenic and noncytotoxic
2) Easy disposal: Safe to dispose in the drain
3) Good Compatibility: Spectrally compatible with all the existing instruments
4) High Sensitivity: Higher signal but lower background
5) High Stability: can be stored at RT and microwavable
Figure 1. Normalized excitation and emission spectra of SafeGreen®(green lines： Absorption λmax=270nm, 505nm；Emission λmax=525 nm) in TE buffer with dsDNA； Normalized excitation and emission spectra of SafeRed®(red lines：Absorption λmax=290nm, 520nm；Emission λmax=590 nm) in TE buffer with dsDNA
Post-staining Protocol
1) Run gels as usual according to your standard protocol.
2) Dilute theSafeGreen®10,000X stock reagent~3,300 fold to make a 3X staining solution in H2O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the staining solution is reused.
3) Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel.
4) Agitate the gel gently at room temperature for~30 minutes.
5) Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR filter or GelStar filter.
6) Staining solution can be reused at least 2~3times. Store staining solution at room temperature protected from light.
Pre-cast Protocol
1) Prepare molten agarose gel solution using your standard protocol.
2) Dilute the DuGreen®10,000X stock reagent into the molten agarose gel solution at 1:10,000 and mix thoroughly. DuGreen®can be added while the gel solution is still hot.
3) Cast the gel and allow it to solidify. Any left over gel solution may be stored and reheated later for additional gel casting. DuGreen® precast gels may be stored at 4°C for later use.
4) Load samples and run the gels using your standard protocol.
5) Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator or a laser-based gel scanner using a long path green filter such as a SYBR filter or FITC filter.
Note: The pre-cast protocol is not recommended for polyacrylamide gels.
NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.
特别提醒：
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！
Description
| Name | SafeGreen Nucleic Acid Gel Stain (~ Sybr Green and GelGreen) | ||
|---|---|---|---|
| CAT# | 011-500uL | CAS# | N/A |
| Storage# | 4°C Sealed & desiccated & Minimized light exposure & Minimized Freezing And Thawing! | Shelf Life# | 24 months |
| Ex(nm)# | 471 | Em(nm)# | 540 |
| MW# | N/A | Solvent# | DMSO |
特别提醒：
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！
产品参数：
| Name | SafeGreen GelStain核酸染料, 10000xGelGreen和DuGreen | ||
|---|---|---|---|
| CAT# | 011-500μL | CAS# | N/A |
| Storage# | 4℃ Sealed & desiccated & Minimized light exposure & Minimized Freezing And Thawing | Shelf Life# | 24 months |
| Ex(nm)# | 471 | Em(nm)# | 540 |
| MW# | N/A | Solvent# | Water |
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