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钙荧光探针bioFluo-3, AM

bioFluo-3, AM是最常用的检测细胞内钙离子浓度的荧光探针之一。它穿透细胞膜进入细胞后被细胞内的酯酶剪切形成Fluo-3，从而被滞留在细胞内，Fluo-3若以游离配体形式存在时几乎是非荧光性的，但是当它与细胞内钙离子结合后可以产生较强的荧光，最大激发波长为506nm，最大发射波长为526nm。实际检测时推荐使用的激发波长为488nm左右，发射波长为525～530nm。可以使用激光共聚焦显微镜或流式细胞仪检测细胞内钙离子浓度的变化。

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浏览量:

1000

产品编号

21010-100ug/365元;1mg/ 1650元

所属分类

Fluorescence reagents and more

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库存:



产品描述

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钙荧光探针bioFluo-3, AM

bioFluo^®为北京富百科生物技术有限公司注册商标，未经授权严禁使用！

CAS#：121714-22-5

中文名：钙荧光探针bioFluo-3, AM也称之为 Fluo-3, AM

英文名：1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’-tetraacetic acid, pentaacetoxymethyl ester

结构式：

分子式：C₅₁H₅₀Cl₂N₂O₂₃分子量：1129.85

储存条件：-20℃干燥避光保存，有效期一年

Fluo-3, AM

Chemical Name: 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]- 2-(2-amino-5-methylphenoxy)ethane-N,N,N E N Etetraacetic acid, pentaacetoxymethyl ester]

Appearance: red solid；HPLC Purity: >90.0 %； MW: C₅₁H₅₀C_l2N₂O₂₃=1129.85； CAS: 121714-22-5

Storage&Shipping:

Store at -20°C protected from light. Product is shipped at ambient temperature.

Discription:

Fluo 3 is a long wavelength calcium probe. Fluo 3 is practically nonfluorescent in its free ligand form, but its fluorescence increases 60-80 times when it forms complexes with calcium. Thus, it has been widely used with confocal laser fluorescent microscopy because the microscope has an argon laser. The long wavelength of the fluorescent signal is also convenient for minimizing photodamage to sample cells. Fluo 3 is also useful for caged calcium and others that are cleaved by the photoirradiation in the UV region. Fluo 3-AM is an acetoxymethyl ester derivative of Fluo 3 that can be easily loaded into cells by incubation.

Protocol for Cell Staining (Human T cells) *

Reagents:

· 2 mM Fluo 3-AM/DMSO (1 mg Fluo 3-AM in 442 μl DMSO)

· Pluronic F127

· Hanks Ebalanced salt solution (HBSS)

· HEPES buffer saline (10 mM HEPES, 1 mM Na2HPO4, 137 mM NaCl, 5 mM KCl, 1 mM CaCl2, 0.5 mM MgCl2, 5 mM glucose, 0.1% BSA, pH 7.4)

Protocol:

1. Add 16.5 mg Pluronic F127 to Fluo 3-AM/DMSO solution. Pluronic F127 prevents aggregation of Fluo 3-AM in HBSS and helps uptake with cells.

2. Dilute the Fluo 3-AM solution with HBSS to prepare 4 μM Fluo 3-AM working solution.

3. Add the Fluo 3-AM working solution to the cells and incubate at 37^oC for 20 min.

4. Add HBSS containing 1% fetal Calf serum equivalent to 5 times the volume of Fluo 3 -AM working solution (step 3).

5. Wash the cells 3 times with HEPES buffer saline. Then resuspend the cells to prepare 1x105 cells per ml solution using HEPES buffer saline.

6. Incubate at 37^oC for 10 min. Then use the cells for fluorescent calcium ion detection.

7. Monitor the fluorescence at 528 nm (excitation: 490-500 nm)

*This protocol should be required for optimal results with an individual cell lines

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅!

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

产品参数

Name	钙荧光探针bioFluo-3, AM
CAT#	22010	CAS#	121714-22-5
Storage#	−20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	506	Em(nm)#	526
MW#	1129.85	Solvent#	DMSO

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