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钙荧光探针bioFluo-4, AM

bioFluo-4是一种将bioFluo-3结构中的Cl替换成F的钙荧光探针。由于将Cl替换成了电子吸引力更强的F，它的最大激发波长会向短波长处偏离10 nm左右。这个波长更接近于氩激光器的波长，所以用氩激光器激发时，Fluo-4的荧光强度比Fluo-3强一倍。Fluo-4, AM穿透细胞膜进入细胞后被细胞内的酯酶剪切形成Fluo-4，从而被滞留在细胞内，Fluo-4若以游离配体形式存在时几乎是非荧光性的，但是当它与细胞内钙离子结合后可以产生较强的荧光，最大激发波长为494nm，最大发射波长为516nm。可以使用激光共聚焦显微镜或流式细胞仪检测细胞内钙离子浓度的变化。

零售价

0.0

元

市场价

0.0

元

浏览量:

1000

产品编号

21130-100ug/475元;1mg/ 2150元

所属分类

Fluorescence reagents and more

数量

库存:



产品描述

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钙荧光探针bioFluo-4, AM

bioFluo^®为北京富百科生物技术有限公司注册商标，未经授权严禁使用！

产品组成：bioFluo-4, AM也之称为Fluo-4, AM

中文名：钙荧光探针bioFluo-4, AM，CAS#：273221-67-3；分子式：C₅₁H₅₀F₂N₂O₂₃=1096.94

英文名：1-[2-Amino-5-(2,7-difluoro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester

bioFluo-4, AM

中文名：钙荧光探针Fluo-4, AM，CAS#：273221-67-3；分子式：C₅₁H₅₀F₂N₂O₂₃=1096.94

英文名：1-[2-Amino-5-(2,7-difluoro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester

bioFluo-4, AM

Cat#	Size	Price	MW	Abs	Em	Soluble in	Storage
21110	1 mg	$175	1096.95	494 nm	518 nm	DMSO	F/D/L

References

1. Hanson BJ. (2006) Multiplexing Fluo-4 NW and a GeneBLAzer transcriptional assay for high-throughput screening of G-protein-coupled receptors. J Biomol Screen, 11, 644.

2. Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR. (2004) Novel fluo-4 analogs for fluorescent calcium measurements. Cell Calcium, 36, 509.

3. Gee KR, Brown KA, Chen WN, Bishop-Stewart J, Gray D, Johnson I. (2000) Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium, 27, 97.

4. Juul Jensen ME, Odgaard E, Christensen MH, Praetorius HA, Leipziger J. (2007) Flow-Induced [Ca2+]i Increase Depends on Nucleotide Release and Subsequent Purinergic Signaling in the Intact Nephron. J Am Soc Nephrol.

5. Kolar SS, Barhoumi R, Lupton JR, Chapkin RS. (2007) Docosahexaenoic Acid and butyrate synergistically induce colonocyte apoptosis by enhancing mitochondrial Ca2+ accumulation. Cancer Res, 67, 5561.

6. Xin H, Wang Y, Todd MJ, Qi J, Minor LK. (2007) Evaluation of No-Wash Calcium Assay Kits: Enabling Tools for Calcium Mobilization. J Biomol Screen.

7. Ban JY, Cho SO, Jeon SY, Bae K, Song KS, Seong YH. (2007) 3,4-Dihydroxybenzoic acid from Smilacis chinae rhizome protects amyloid beta protein (25-35)-induced neurotoxicity in cultured rat cortical neurons. Neurosci Lett, 420, 184.

8. Hashimoto T, Ohata H, Nobe K, Sakai Y, Honda K. (2007) A novel approach for the determination of contractile and calcium responses of the basilar artery employing real-time confocal laser microscopy. J Pharmacol Toxicol Methods, 56, 79.

9. Braun T, Voland P, Kunz L, Prinz C, Gratzl M. (2007) Enterochromaffin cells of the human gut: sensors for spices and odorants. Gastroenterology, 132, 1890.

10. Lee HT, Hennig GW, Fleming NW, Keef KD, Spencer NJ, Ward SM, Sanders KM, Smith TK. (2007) The mechanism and spread of pacemaker activity through myenteric interstitial cells of cajal in human small intestine. Gastroenterology, 132, 1852.

11. Raye WS, Tochon-Danguy N, Pouton CW, Haynes JM. (2007) Heterogeneous population of dopaminergic neurons derived from mouse embryonic stem cells: preliminary phenotyping based on receptor expression and function. Eur J Neurosci, 25, 1961.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅!

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

产品参数

Name	钙荧光探针bioFluo-4, AM
CAT#	21130	CAS#	273221-67-3
Storage#	−20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	494	Em(nm)#	516
MW#	1096.94	Solvent#	DMSO

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