

Annexin V-FITC/PI 细胞凋亡检测试剂盒

The Annexin V-FITC Apoptosis assay kit detects apoptotic cells by flow cytometry. The annexins are a group of homologous proteins which bind phospholipids in the presence of calcium. Annexin V-FITC is a fluorescent probe which binds to phosphatidylserine in the presence of calcium. Apoptosis, or programmed cell death, is a mechanism of cells used to negatively select cells that are deleterious to the host. The cellular changes involved in the process include loss of phospholipid asymmetry during the early stages of apoptosis. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg-ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidyl- serine, which is normally found on the internal part of the plasma membrane, becomes translocated to the external portion of the membrane. The phosphatidyl- serine becomes available to bind to the annexin V-FITC conjugate in the presence of calcium. The procedure consists of the binding of annexin V- FITC to phosphatidylserine in the membrane of cells, which are beginning the apoptotic process, and the binding of propidium iodide to the cellular DNA in cells where the cell membrane has been totally compromised.

零售价

0.0

元

市场价

0.0

元

浏览量:

1000

产品编号

2020-20T/400元50T/800元,100T/1200元

所属分类

Fluorescence reagents and more

数量

库存:



产品描述

[[[[[[[[[[[[[[[[[[[[[[[[产品参数, 参数]]]]]]]]]]]]]]]]]]]]]]]]

Annexin V-FITC/PI Dead Cell Apoptosis Detection Kit

Product Description

Reagents:

Reagents	20 assays	50 assays	100 assays	Storage
Annexin V-FITC	100 μl	250 μl	500 μl	4°C in the dark
Propidium Iodide, PI	200 μl	500 μl	1000 μl	4°C in the dark
Binding Buffer ( 4´ )	4 ml	10 ml	20 ml	4°C

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Annexin V is a product of human origin; handle as if capable of transmitting infectious agents.

Preparation Instructions

Allow all kit components to reach room temperature before use.

Prepare 1× Binding Buffer by diluting 1 ml of the 4× Binding Buffer with 3 ml of deionized water.

Dissolve the staurosporine in DMSO to a concentration of 100 μg/ml.

Procedure

This procedure describes the induction of apoptosis in the Jurkat cell line, followed by measurement of phosphatidylserine. Perform the experiment using aseptic technique.

1. Induce apoptosis in a 1 x 10⁶ cells/ml suspension of Jurkat cells by the addition of 1 μg/ml staurosporine.

2. Establish a control of non-induced Jurkat cells at 1 x 10⁶ cells/ml for a zero time data point.

3. Incubate both Jurkat cell cultures for 1–2 hours in a 37 °C, 5% CO₂ incubator.

4. Wash the cells twice with DPBS.

5. Resuspend the cells in 1× Binding Buffer at a concentration of ~1 x 10⁶cells/ml.

6. Add 500 μl of the apoptotic cell suspension to a plastic 12 × 75 mm test tube.

7. Add 500 μl of the non-induced cell suspension to a second plastic 12 × 75 mm test tube.

8. Add 5 μl of Annexin V FITC Conjugate and 10 μl of Propidium Iodide Solution to each cell suspension.

9. Incubate the tubes at room temperature for exactly 10 minutes and protect from light.

10. Determine the fluorescence of the cells immediately with a flow cytometer. Cells, which are early in the apoptotic process, will stain with the Annexin V FITC Conjugate alone. Live cells will show no staining by either the Propidium Iodide Solution or Annexin V FITC Conjugate. Necrotic cells will be stained by both the Propidium Iodide Solution and Annexin V FITC Conjugate.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	Annexin V-FITC/PI Dead Cell Apoptosis Detection Kit
CAT#	2020	CAS#	N/A
Storage#	-20 °C Sealed & desiccated & Minimized light exposure & Minimized Freezing And Thawing!	Shelf Life#	24 months
Ex(nm)#	490	Em(nm)#	520
MW#	N/A	Solvent#	DMSO

扫二维码用手机看

上一个

Annexin V-Super Fluor 488/Dead Cell Apoptosis Detection Kit

下一个

PKH26红色荧光细胞链接试剂盒 PKH26 Red Fluorescent Cell Linker Kit

Annexin V-FITC/PI 细胞凋亡检测试剂盒

相关企业下载