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FBGreen dsDNA for Qubit定量检测试剂盒（～PicoGreen）

The FBKGreen reagent is an asymmetrical cyanine dye. Free dye does not fluoresce, but upon binding to dsDNA it exhibits a >1000-fold fluorescence enhancement. PicoGreen is 10000x more sensitive than UV absorbance methods, and highly selective for dsDNA over ssDNA and RNA.

零售价

0.0

元

市场价

0.0

元

浏览量:

1000

产品编号

015-1mL/3950 元; 0.1mLx10/4150元

所属分类

Qubit reagents

数量

库存:



产品描述

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FBKGreen dsDNA HS Assay Kit (~PicoGreen)

Contents and Storage Information：

Components	100assays Q32851	500 assays Q32854	Concentration	Storage and Stability
FBKGreen dsDNA HS Reagent (Component A)	250μL	1.25mL	200X in DMSO	4^oC Protect from light 6 months
dsDNA HS Buffer (Component B)	50mL	2500mL	N/A
dsDNA HS Standard#1 (Component C)	1mL	5mL	0 ng/uL in TE Buffer
dsDNA HS Standard#2(Component D)	1mL	5mL	10 ng/uL in TE Buffer

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

Turn on the fluorescence plate reader at least 10 minutes before reading results. Use the following settings to read the PicoGreen results:

Wavelength/bandwidth

Excitation: ~485nm/20nm

Emission: ~530nm/25nm

Prepare 1X TE buffer (10mM Tris-HCl, 1mM EDTA, pH 7.5) from the 20X TE stock, which is supplied in the PicoGreen kit (to make 50mL, add 2.5mL of 20X TE to 47.5mL sterile distilled DNase-free water). 50mL is sufficient for 250 assays.
Dilute DNA standards form 100μg/mL to 2μg/mL with 1X TE. For two standard curves prepare 600μL of a 2μg/mL stock by adding 12μL of the 100μg/mL stock to 588μL 1X TE.
Prepare the two standard curves in the microtiter plate as shown in the table:

Plate Well	Final [DNA] (ng/mL)	Vol. (μL) 2mg/mL DNA standard	Vol.( μL) 1X TE buffer	Final Total DNA (ng)/Well
Any	0	0	100	0
A1 & A2	25	2.5	97.5	5
B1 & B2	50	5	95	10
C1 & C2	100	10	90	20
D1 & D2	200	20	80	40
E1 & E2	300	30	70	60
F1 & F2	400	40	60	80
G1 & G2	500	50	50	100
H1 & H2	1000	100	0	200

For each unknown, add 1μL of sample to 99μL of 1X TE in the microplate well. Mix by pipetting up and down.
Prepare a 1:200 dilution of the PicoGreen reagent in 1X TE. For each standard and each unknown sample, a volume of 100μL will be needed. For examples, 2 standard curves with 8 points each will require 1.6mL. To calculate the total volume of diluted PicoGreen reagent needed, determine the total number of samples and unknowns you will be testing and multiply this number by 100μL (if using a multichannel pipet, make extra reagent). The PicoGreen reagent is light sensitive and should be kept wrapped in foil while thawing and in the diluted state. Vortex well.
Add 100μL of diluted PicoGreen to every standard and sample. Mix by pipetting up and down.
Cover the microplate with foil and allow to incubate at room temperature for 2-5 minutes.
Read the plate.
Generate a standard curve using the average values of the standards and determine the concentrations of DNA in the unknown samples.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅！

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	FBGreen dsDNA for Qubit定量检测试剂盒（～PicoGreen）
CAT#	015-1mL; 015-0.1mLx10	CAS#	N/A
Storage#	-20°C Sealed & desiccated & Minimized light exposure & Minimized Freezing And Thawing!	Shelf Life#	24 months
Ex(nm)#	488	Em(nm)#	520
MW#	N/A	Solvent#	DMSO

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上一个

Qubit dsDNA HS Assay Kit 荧光定量检测试剂盒Qubit dsDNA HS(高灵敏度)

下一个

FBK dsDNA BR Assay Kit for Qubit荧光定量检测试剂盒 Qubit dsDNA BR(宽范围)

FBGreen dsDNA for Qubit定量检测试剂盒（～PicoGreen）

Wavelength/bandwidth

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