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Femto ECL Substrate 特超敏发光液

1800元/套(50mL+50mL); 5800元/套(250mL+250mL)
The Fluorescence West Femto ECL Substrate is an extremely sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. This substrate’s extremely intense signal output enables detection of low femtogram amounts of antigen. Furthermore, this substrate requires substantial dilutions of the primary and secondary antibodies. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots also can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

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市场价

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浏览量:

1000

产品编号

046-100mL/1800元(50mL A液+50mL B液)

所属分类

ECL Western Blotting Substrate

数量

库存:



产品描述

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Femto ECL^®Western Blotting Substrate

DuraECL®, FemtoECL®，PicoECL®，ECLplus®are registered trademarks and strictly forbidden to be used without authorization.

Kit Contents: ECL Solution A, 10mL; ECL Solution B, 10mL; Sufficient for 200cm²of membrane

Storage: Upon receipt store solutions at 4°C stable for 12 months. These products are shipped at RT.

Introduction

The Fluorescence Femto ECL Substrate is an extremely sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. This substrate’s extremely intense signal output enables detection of low femtogram amounts of antigen. Furthermore, this substrate requires substantial dilutions of the primary and secondary antibodies. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots also can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

Procedure Summary

Note: The recommended range of antibody dilutions must be used to obtain positive results. The optimal antigen and antibody amounts to use may require experimentation.

1. Dilute primary antibody to 5-100ng/mL.

2. Dilute secondary antibody to 2-10ng/mL.

3. Mix the two-substrate components at a 1:1 ratio to prepare the substrate Working Solution.

4. Incubate blot 5 minutes in West Femto ECL Substrate Working Solution.

5. Drain excess reagent. Cover blot with clear plastic wrap.

6. Expose blot to X-ray film or Use imaging devices.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

不同ECL发光液检测灵敏度

产品名称	检测浓度	推荐一抗孵育浓度（ng/mL）	推荐二体孵育浓度（ng/mL）
ECL plus	皮克级~10^-12g	一抗浓度：100～500	二抗浓度：10～50
Pico ECL	皮克级至飞克级10^-12g～10^-15g	一抗浓度：10～200	二抗浓度：5～50
Femto ECL	小于飞克级<10^-15g	一抗浓度：2～100	二抗浓度：2～20
Dura ECL	小于飞克级<10^-15g	一抗浓度：1～100	二抗浓度：1～10

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

产品参数

Name	Femto ECL®特超敏发光液
CAT#	046-100mL; 046-500mL	CAS#	N/A
Storage#	4℃ Sealed & desiccated & Minimized light exposure	Shelf Life#	12 months
Ex(nm)#	N/A	Em(nm)#	N/A
MW#	N/A	Solvent#	N/A

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