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SafeStain® GelStain® Nucleic Acid Gel Stain(>SafeRed)
SafeStain® Nucleic Acid Gel Stain
SafeStain® is the registered trademark and strictly forbidden to be used without authorization.
Description
SafeStain® Nucleic Acid Gel Stain is our 4th generation of fluorescent nucleic acid gel stain designed to replace GelRed, GelGreen, Sybr Safe, Sybr Gold and the highly toxic ethidium bromide (EtBr). The Ames test confirmed that are nonmutagenic at concentrations well above their working concentrations used for gel staining. Nucleic Acid Gel Stain is highly sensitive than EtBr either as precast gel stains or post gel stains. SafeStain is suitable for all the existing electrophoresis imaging system including standard transilluminator(280～380nm) and Blue light instrument (similar visible light 450～500nm) without changing your existing imaging system. SafeStain® can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Precast polyacrylamide gel staining with SafeStain® is not recommended because of relatively high background fluorescence. Furthermore, Polyacrylamide gel staining with SafeStain can be watched by the eyes.
SafeStain® Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated ® solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial (0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.
Gel staining with SafeStain® is compatible with downstream applications such as gel extraction and cloning. SafeStain® is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Features
- Safety: Nonmutagenic and noncytotoxic
- Easy disposal: Safe to dispose in the drain
- Compatibility: Spectrally compatible all the existing instruments including standard transilluminator(280～380nm) and Blue light instrument (similar visible light 450～500nm)
- Sensitivity: Higher signal but lower background
- Stability: can be stored at RT and microwavable
1.Post-staining Protocol
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- Run gels as usual according to your standard protocol.
- Dilute the SafeStain® 10,000X stock reagent~3,300 fold to make a 1X staining solution in H2O with 0.1M Nacl(e.g, add 5ul of SafeStain® 10,000X stock reagent and 5ml 1M Nacl to 45ml H2O). Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.
- Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X staining solution to submerge the gel.
- Agitate the gel gently at room temperature for~30 minutes.
- Image the stained gel with a standard transilluminator (302 or 312nm), and photograph the gel using an ethidium bromide filter.
- Staining solution can be reused at least 2~3times. Store staining solution at room temperature protected from light.
2. Pre-cast Protocol
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- Prepare molten agarose gel solution using your standard protocol.
- Dilute the SafeStain® 10,000X stock reagent into the molten agarose gel solution at 1:10,000 (e.g.,5ul of SafeStain® 10,000Xstock reagent added to 50ml of the gel solution)and mix thoroughly. can be added while the gel solution is still hot.
- Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting. SafeStain® precast gels may be stored at 4°C for later use.
- Load samples and run the gels using your standard protocol.
- Image the stained gel with a standard transilluminator(302 or 312nm), and photograph the gel using an Ethidium bromide filter.
Note: The pre-cast protocol is not recommended for polyacrylamide gels. Although the post-staining method is recommended, precast gels may also be tried with SafeStain®. However, some DNA samples, such as those derived from plasmid DNA digestion by certain restriction enzymes, may experience migration retardation or compromised resolution. Thus, both the post-stained and precast gels can be performed to determine which one may better meet your needs.
SafeStain® troubleshooting
1. Why am I seeing smeared or smiling DNA band(s) or discrepant DNA migration?
Try reducing the amount of DNA loaded. The recommended loading amount for ladders and samples of known concentration is 50-200 ng/lane. For samples of unknown concentration, try loading one half or one third of the usual amount of DNA. This usually solves band migration problems.. Blown out or smeared bands can be caused by overloading. This is frequently observed with DNA ladders.
Description
| Name | SafeStain® GelStain® Nucleic Acid Gel Stain(>SafeRed) | |||
|---|---|---|---|---|
| CAT# | 088-500uL | CAS# | N/A | |
| Storage# | 4℃ Sealed & desiccated & Minimized light exposure &Minimized Freezing And Thawing | Shelf Life# | 24 months | |
| Ex(nm)# | 290nm，304nm，480nm，520nm | Em(nm)# | 520nm，590nm | |
| MW# | Solvent# | |||
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