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Super Green I for PCR (20× in DMSO)(~Sybr Green)

Super Green I is the ideal substitute for Sybr Green, Super Green is a very sensitive dye for the detection of double stranded DNA (dsDNA), So it has been widely used in non-specific detection of amplification in realtime qPCR experiments. The double-strand DNA-specific SYBR Green I fluorescent reporter offers distinct advantages. SYBR Green I dye is inexpensive, easy to use, and sensitive. Well-designed primers must be used in SYBR Green quantitative RT-PCR reactions because SYBR Green I dye will detect nonspecific products, resulting in an overestimation of the target concentration.

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1000

产品编号

003-500μL/120元; 1mL/215元

所属分类

qPCR reagents

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

产品描述

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Super Green I for PCR

1. The flowing table 1 is our SYBR Green I RT-PCR Reagents Kit recipe for reference only. Please optimize it by yourself.

Reagent	Final concentration in the mix
dNTP	0.25mM
Tween20	1%
BSA	0.1% vol.
Tris(pH8.4)	50mM
Hot-start Taq DNA polymerase	1.25 u per reaction
NH₄Cl	10mM
KCl	20mM
MaCl₂	2.5mM
Sybr Green	2X

2. On ice, prepare a 2x master mix containing no DNA, by mixing the components in the following order: water, DMSO, Taq polymerase buffer, dNTPs, MgCl2, Sybr Green, Taq polymerase.

3. Transfer 2x QuantiTect SYBR Green PCR Master Mix to tubes or plates. Add RNase-free water. Mix the individual solutions.

4. Prepare a reaction mix according to Table 2.

Due to the hot start, it is not necessary to keep samples on ice during reaction setup or while programming the real-time cycler.

PCR Reaction Setup: Table 2

DNA	Template DNA (<500 ng/reaction)
SYBR Mix	25.0 μL
Primer1（引物1）	2ul（5uM)
Primer2（引物2）	2ul（5uM）
dd H2O	Added to 50.0 μL

Program your real-time cycler according to the program outlined in Table 3

PCR initial activation step

Heating cycling

Number of cycles 40

95 °C 5min

96 °C 10s，

60 °C 30s

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅！

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	Super Green I for PCR (20× in DMSO)(~Sybr Green)
CAT#	003-500uL; 003-1mL	CAS#	N/A
Storage#	-20°C Sealed & desiccated & Minimized light exposure & Minimized Freezing And Thawing!	Shelf Life#	24 months
Ex(nm)#	497	Em(nm)#	525
MW#	N/A	Solvent#	DMSO

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