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6-CR6G; 5-CR6G; 5,6-CR6G
CR6G is the purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. This isomer is predominantly used to label small molecules, peptides and proteins.
6-CR6G [6-Carboxyrhodamine 6G, hydrochloride]
|
Cat# |
Size |
Price |
MW |
Abs |
Em |
Soluble in |
Storage |
|
1003 |
5mg |
¥1050 |
494.97 |
520 nm |
547 nm |
DMSO |
4ºC |
|
|
Features and Biological Applications6-CR6G is the other purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Complimentary to 5-CR6G, 6-CR6G is predominantly used for labeling nucleotides and nucleic acids.
|
5-CR6G [5-Carboxyrhodamine 6G, hydrochloride]
|
Cat# |
Size |
Price |
MW |
Abs |
Em |
Soluble in |
Storage |
|
1002 |
10 mg |
¥1050 |
494.97 |
518 nm |
544 nm |
DMSO |
4ºC |
|
|
Features and Biological Applications5-CR6G is the purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. This isomer is predominantly used to label small molecules, peptides and proteins.
|
5(6)-CR6G [5-(and-6)-Carboxyrhodamine 6G, hydrochloride]
|
Cat# |
Size |
Price |
MW |
Abs |
Em |
Soluble in |
Storage |
|
1001 |
25 mg |
¥1195 |
494.97 |
519 nm |
544 nm |
DMSO |
4ºC |
|
|
Features and Biological Applications5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.
|
References
1. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci USA, 2005, 102, 5926-31.
2. Hung, S.C., et al., Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem,1996, 238, 165-70.
3. Chiu, D.T., et al., Injection of ultrasmall samples and single molecules into tapered capillaries. Anal Chem, 997. 69, 1801-7.
4. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem1997. 252, 78-88.
5. Hung SC, et al. (1996). Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem238, 165-70.
6. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides.Proc Natl Acad Sci U S A2005, 102, 5926-31.
7. Dietrich, A., et al., Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: A comparative study of ensemble and single-molecule data.J Biotechnol2002, 82, 211-31.
8. Arezi, B., et al., Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.J Mol Biol2002, 322, 719-29.
9. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem252, 78-88.
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Description
| Name | 5(6)-CR6G [5-(and-6)-Carboxyrhodamine 6G, hydrochloride] | ||
|---|---|---|---|
| CAT# | 330-25mg | CAS# | |
| Storage# | -20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 519 | Em(nm)# | 544 |
| MW# | 494.97 | Solvent# | DMSO |
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