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FAM
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction.
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产品编号
106(6-FAM)-103(5-FAM)-100(56FAM)
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库存:
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1
产品描述
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FAM (Carboxyfluorescein)
性质：
1) 外观：黄色粉末; 2)纯度：≥95%（HPLC）; 3) MW：C21H12O7=376.32
储存条件：-20℃干燥避光保存，有效期两年。
产品描述：用于标记蛋白、多肽、抗体及Oligo
英文名：6-FAM [6-Carboxyfluorescein] *Single isomer*, 中文名：6-羧基荧光素 CAS#：3301-79-9
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Features and Biological Applications6-FAM is the other purified isomer of 5(6)-carboxyfluorescein. Complimemtary to 5-FAM isomer, 6-FAM is mainly used in labeling nucleotides and nucleic acids. |
5-FAM [5-Carboxyfluorescein] CAS#76823-03-5
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Features and Biological Applications5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules. |
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS#72088-94-9
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Features and Biological ApplicationsCarboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications. |
References
1. Brandis JW (1999). Dye structure affects Taq DNA polymerase terminator selectivity. Nucleic Acids Res27, 1912-8.
2. Witham PK,et al. (1996). A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef. Appl Environ Microbiol62, 1347-53.
3. Hahn M, et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis22, 2691-700.
4. Hung SC, et al. (1996). Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers. Anal Biochem243, 15-27.
5. Banks PR and Paquette DM (1995). Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis. Bioconjug Chem6, 447-458.
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1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
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产品参数
| Name | FAM,Carboxyfluorescein | ||
|---|---|---|---|
| CAT# | 106(6-FAM)-103(5-FAM)-100(5,6FAM) | CAS# | |
| Storage# | -20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 495 | Em(nm)# | 517 |
| MW# | 376.32 | Solvent# | DMSO |
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