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TAMRA SE
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction.
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产品编号
376(6-TAMRA SE)-373(5-TAMRA SE)
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1
产品描述
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TAMRA, SE [Carboxytetramethylrhodamine, succinimidyl ester]
TAMRA® is the registered trademark and strictly forbidden to be used without authorization.
Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction:
1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).
2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.
3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.
4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#150810-69-8
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Features and Biological Applications6-TAMRA, SE is the other purified single isomer of 5(6)-TAMRA, SE. It is predominantly used for nucleotide labeling and DNA sequencing.
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5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#150810-68-7
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Features and Biological Applications5-TAMRA, SE is predominantly used for labeling peptides and proteins while 6-TAMRA, SE is often used for labeling nucleotides and sequencing nucleic acids. The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. |
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] CAS#246256-50-8
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Features and Biological ApplicationsThe succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have also been widely used as acceptors for FAM fluorophores in a variety of FRET studies. |
References
1. Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.
2. Nasarabadi S, et al.(1999). Simultaneous detection of TaqMan probes containing FAM and TAMRA reporter fluorophores. Biotechniques27, 1116-8.
特别提醒：
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！
Description
| Name | TAMRA, SE [Carboxytetramethylrhodamine, succinimidyl ester] | ||
|---|---|---|---|
| CAT# | 376(6-TAMRA SE)-373(5-TAMRA SE)-370(56-TAMRA SE) | CAS# | |
| Storage# | -20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 547 | Em(nm)# | 575 |
| MW# | 527.53 | Solvent# | DMF or DMSO |
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