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SuperFluor 488 Phalloidin标记鬼笔环肽 Actin-Tracker Green-488 (微丝绿色荧光探针)
鬼笔环肽（Phalloidin）是一种来源于毒蕈类鬼笔鹅膏（Amanita phalloides）的环状七肽毒素，以高亲和力（Kd= 20 nM） 选择性结合于丝状肌动蛋白 F-actin，而不会与单体肌动蛋白 G-actin 结合。鬼笔环肽衍生物也以相近的亲和力结合于大小纤维，无论是动植物来源的肌肉细胞或非肌肉细胞，按照每一个肌动蛋白亚基约与一个鬼笔环肽分子的计量比结合， 且非特异性结合几乎可忽略，染色区域和非染色区域辨识度非常明显，因此，鬼笔环肽衍生物特别适合替代肌动蛋白（Actin）抗体进行相关研究。另外，鬼笔环肽（Phalloidin）的结合可以阻止丝状肌动蛋白（微丝）的解离，稳定微丝结构，从而破坏微丝的聚合-去聚合的动态平衡。此特性使得肌动蛋白聚合发生的临界浓度（CC）降至＜1 µg/mL，因此，可用作一种聚合促进剂。此外，鬼笔环肽还可抑制 F-actin 的 ATP 水解活性。
PKH67 绿色细胞膜染色试剂盒 PKH67 Green Fluorescent Cell Linker Mini Kit
荧光染料PKH67 适⽤于常规细胞膜标记，是⼀种可对体外和体内细胞示踪的绿⾊荧光染料，通过与膜结构的脂质分⼦结合⽽标记细胞。
主要⽤于细胞体外标记、体外细胞增殖研究以及体内外的细胞示踪研究。PKH67的体内荧光半衰期为10-12天。相⽐于PKH-67，PKH-26具有更⻓的半衰期，标记在兔红细胞上的PKH26半衰期⻓达100天以上。特别适⽤于体外增殖研究以及⻓期的体内细胞跟踪研究。PKH67标记细胞后通常⽤流式细胞仪进⾏细胞增殖检测。
主要⽤于细胞体外标记、体外细胞增殖研究以及体内外的细胞示踪研究。PKH67的体内荧光半衰期为10-12天。相⽐于PKH-67，PKH-26具有更⻓的半衰期，标记在兔红细胞上的PKH26半衰期⻓达100天以上。特别适⽤于体外增殖研究以及⻓期的体内细胞跟踪研究。PKH67标记细胞后通常⽤流式细胞仪进⾏细胞增殖检测。
DRAQ5
The DRAQ5® Fluorescent Probe is a far-red dye for cell stain in live or fixed cells, a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ5 can be multiplexed with many other fluorophores
DRAQ7
DRAQ7® is a bright, easy-to-use far-red fluorescent dye. DRAQ7® Dye is a membrane-impermeable dye that rapidly stains the dead or fixed cells for cell cycle analysis. Because DRAQ7® is impermeable to intact cells, it may also be used as a viability dye.
Calcein, AM
Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to Calcein. After Calcein-AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell (Fig. 1). Among other reagents, including BCECF-AM and Carboxy-fluorescein diacetate, Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any cellular functions such as proliferation or chemotaxis of lymophocyte. In addition, viability assays using Calcein are reliable and correlate well with the standard 51Cr-release assay. The excitation and emission wavelengths of calcein are 490 nm and 515 nm, respectively.
JC-10
JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have also developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1.
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