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ECL Plus®高敏发光液

500元/套(50mL+50mL); 2000元/套(250mL+250mL)
The ECL plus Substrate is a highly sensitive nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL plus Substrate enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed. The optimally formulated ECL plus Substrate makes it the ideal substitute for other similar substrates without the need for additional optimization of assay conditions.

零售价

0.0

元

市场价

0.0

元

浏览量:

1000

产品编号

044-100mL/500元(50mL A液+50mL B液)

所属分类

ECL Western Blotting Substrate

数量

库存:



产品描述

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ECL Plus^® Western Blotting Substrate

DuraECL®, FemtoECL®，PicoECL®，ECLplus®are registered trademarks and strictly forbidden to be used without authorization.

Kit Contents: ECL plus Solution A, 50 mL; ECL plus Solution B, 50 mL; sufficient for 1000 cm2 of membrane

Storage:Upon receipt store solutions at 4°C. When stored at room temperature, substrate components are stable for six months. These products are shipped at ambient temperature.

Important Notice: Please use the same blotting conditions when switching from other similar ECL substrates. The ECL plus has been developed to be more sensitive compared to other similar ECL substrates. Careful titration of antigen and antibody concentrations is recommended.

Introduction

The ECL plus Substrate is a highly sensitive nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL plus Substrate enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed. The optimally formulated ECL plus Substrate makes it the ideal substitute for other similar substrates without the need for additional optimization of assay conditions.

Procedure Summary

Note: The recommended range of antibody dilutions should be used as references to obtain positive results. The optimal antigen and antibody amounts to be used may require experimentation.

1. Diluteprimaryantibodyto100-500ng/mL.

2. Dilutesecondaryantibodyto10-50ng/mL.

3. Mixthetwo substratecomponents(AandB)ata1:1ratiotopreparethesubstrateWorkingSolution.

4. Incubateblot5minutesinECL plusSubstrateWorkingSolution.

5. Drainexcessreagent.Coverblotwithclearplasticwrap.

6. ExposeblottoX-rayfilmorUseimagingdevices.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

不同ECL发光液检测灵敏度

产品名称	检测浓度	推荐一抗孵育浓度（ng/mL）	推荐二体孵育浓度（ng/mL）
ECL plus	皮克级~10^-12g	一抗浓度：100～500	二抗浓度：10～50
Pico ECL	皮克级至飞克级10^-12g～10^-15g	一抗浓度：10～200	二抗浓度：5～50
Femto ECL	小于飞克级<10^-15g	一抗浓度：2～100	二抗浓度：2～20
Dura ECL	小于飞克级<10^-15g	一抗浓度：1～100	二抗浓度：1～10

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

Description

Name	ECL Plus Western Blotting Substrate
CAT#	044-100mL; 044-500mL	CAS#	N/A
Storage#	Store in refrigerator (2°C to 8°C)& Sealed & desiccated & Minimized light exposure	Shelf Life#	12 months
Ex(nm)#	N/A	Em(nm)#	N/A
MW#	N/A	Solvent#	N/A

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