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Super Green qPCR mix (~Sybr Green qPCR mix，None/Low/High ROX & Universal)
Super Green qPCR Master Mix is a ready-to-use cocktail containing all components except primers and template.
The 2X master mix contains Taq DNA polymerase, dNTPs, MgCl2, SYBR Green I, Rox or No Rox and stablizers. In the formulation, for HotStart, Taq DNA
Polymerase is chemically modified and its activity is completely blocked until the first denaturation step in PCR program.
This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency. For easy and avoiding potential error manipulation, the products are provided in three formats: no Rox, low Rox, high Rox.
The 2X master mix contains Taq DNA polymerase, dNTPs, MgCl2, SYBR Green I, Rox or No Rox and stablizers. In the formulation, for HotStart, Taq DNA
Polymerase is chemically modified and its activity is completely blocked until the first denaturation step in PCR program.
This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency. For easy and avoiding potential error manipulation, the products are provided in three formats: no Rox, low Rox, high Rox.
RTGreen (～EvaGreen, SYBR Green I, LC Green)
RTGreen (the ideal substitute for Sybr Green and EvaGreen) is a very sensitive dye for the detection of double stranded DNA (dsDNA). The dye is a green fluorescent nucleic acid dye with features that make it useful for non-specific detection of amplification in realtime qPCR experiments. Compared with the widely used SYBR Green I, RTGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification , RTGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in more robust PCR signal. The PCR reaction can be monitored using our existing optical setting for SYBR Green I or FAM on any commercial real-time PCR cycler.
ROX Reference Dye
ROX Reference Dye is used to normalize real-time RT-PCR and PCR assays. It provides an internal reference to which the reporter dye signal can be normalized. This signal normalization is necessary to correct for well-to-well signal fluctuations, often due to the design of the instrument.
100X通用ROX参比染料
Super Green（~Sybr Green）嵌合荧光法进行Real Time PCR的专用试剂
为 2× 预混液。只需加入模板、引物和水，可减少操作步骤，缩短加样时间，降低污染几率, 具有灵敏度高、特异性强、稳定性好等特点。
其核心组分是抗体修饰的热启动 Taq DNA 聚合酶，配合精心优化的Buffer体系，有效抑制非特异性扩增，可对宽广浓度范围的模板进行准确定量，获得稳定可靠的 qPCR 结果
可在宽广的定量区域内得到良好的标准曲线，对目的基因进行准确定量检测，重复性好，可信度高。
为 2× 预混液。只需加入模板、引物和水，可减少操作步骤，缩短加样时间，降低污染几率, 具有灵敏度高、特异性强、稳定性好等特点。
其核心组分是抗体修饰的热启动 Taq DNA 聚合酶，配合精心优化的Buffer体系，有效抑制非特异性扩增，可对宽广浓度范围的模板进行准确定量，获得稳定可靠的 qPCR 结果
可在宽广的定量区域内得到良好的标准曲线，对目的基因进行准确定量检测，重复性好，可信度高。
Super Green I for PCR (20× in DMSO)(~Sybr Green)
Super Green I is the ideal substitute for Sybr Green, Super Green is a very sensitive dye for the detection of double stranded DNA (dsDNA), So it has been widely used in non-specific detection of amplification in realtime qPCR experiments. The double-strand DNA-specific SYBR Green I fluorescent reporter offers distinct advantages. SYBR Green I dye is inexpensive, easy to use, and sensitive. Well-designed primers must be used in SYBR Green quantitative RT-PCR reactions because SYBR Green I dye will detect nonspecific products, resulting in an overestimation of the target concentration.
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